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PE-Cyanine7 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE-Cyanine7 Fluorescent Dye

CloneNo.

17A2

Cat no : PY7-65077

Print datasheet

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

验证数据展示

  • FC experiment of mouse splenocytes using PY7-65077

    FC experiment of mouse splenocytes using PY7-65077

    1x10^6 mouse splenocytes were surface stained with 0.2 ug PE-Cyanine7 Anti-Mouse CD3 (17A2) (PY7-65077, Clone:17A2) or 0.2 ug Isotype Control, and 0.2 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PY7-65077 targets CD3 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen γδTCR-positive T-T hybridoma D1 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD3 antigen, epsilon polypeptide
Synonyms AI504783, CD3, Cd3e, CD3epsilon, T3e
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
Conjugate PE-Cyanine7 Fluorescent Dye
Excitation/Emission Maxima Wavelengths450-500 nm / 778 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for PE-Cyanine7 CD3 antibody PY7-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols