验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PY7-65077 targets CD3 in FC applications and shows reactivity with Mouse samples.
经测试应用 | FC Application Description |
经测试反应性 | Mouse |
免疫原 | γδTCR-positive T-T hybridoma D1 种属同源性预测 |
宿主/亚型 | Rat / IgG2b, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | CD3 antigen, epsilon polypeptide |
别名 | AI504783, CD3, Cd3e, CD3epsilon, T3e |
GenBank蛋白编号 | BC098236 |
基因名称 | CD3ε |
Gene ID (NCBI) | 12501 |
RRID | AB_3674030 |
偶联类型 | PE-Cyanine7 Fluorescent Dye |
最大激发/发射波长 | 450-500 nm / 778 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P22646 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
Product Specific Protocols | |
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FC protocol for PE-Cyanine7 CD3 antibody PY7-65077 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |