CoraLite® Plus 488 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65077

产品说明书

CloneNo. 17A2

宿主/亚型

Rat / IgG2b, kappa

种属反应性

Mouse and More (1)

应用

IF/ICC, FC

AI504783, CD3, Cd3e, CD3epsilon, T3e

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65077-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

CL488-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用FC
经测试反应性 Mouse
文献引用反应性mouse
免疫原 γδTCR-positive T-T hybridoma D1 种属同源性预测
宿主/亚型 Rat / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD3 antigen, epsilon polypeptide
别名 AI504783, CD3, Cd3e, CD3epsilon, T3e
GenBank蛋白编号BC098236
基因名称 CD3ε
Gene ID (NCBI) 12501
RRIDAB_2883163
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP22646
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
FC protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Int J Biol Macromol

Polysaccharide-mediated combination therapy enhances anti-tumor effects by promoting the immune cycle of immunogenic cell death

Authors - Xiang-Long Yu
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