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验证数据展示

  • FC experiment of mouse splenocytes using CL488-65077

    FC experiment of mouse splenocytes using CL488-65077

    1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65077

    IF Staining of mouse splenocytes using CL488-65077

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3 (CL488-65077, Clone: 17A2) at dilution of 1:500.

CoraLite® Plus 488 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65077

Print datasheet

CloneNo. 17A2

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF/ICC, FC

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65077-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Mouse
Immunogen γδTCR-positive T-T hybridoma D1 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD3 antigen, epsilon polypeptide
Synonyms AI504783, CD3, Cd3e, CD3epsilon, T3e
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
RRIDAB_2883163
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
FC protocol for CL Plus 488 CD3 antibody CL488-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols