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CoraLite®594 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

390

Cat no : CL594-65058

Print datasheet

Synonyms

Pecam1, PECAM 1, Pecam, 390

验证数据展示

  • FC experiment of C57BL/6 mouse bone marrow cells using CL594-65058

    FC experiment of C57BL/6 mouse bone marrow cells using CL594-65058

    1x10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD31 (390) (CL594-65058, Clone: 390) or 0.5 ug CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3), and 0.5 ug APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

  • IF Staining of bEnd.3 using CL594-65058

    IF Staining of bEnd.3 using CL594-65058

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite®594 CD31 antibody (CL594-65058, Clone: 390 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inbEnd.3 cells
Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65058-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity mouse
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name platelet/endothelial cell adhesion molecule 1
Synonyms Pecam1, PECAM 1, Pecam, 390
GenBank Accession NumberBC008519
Gene Symbol CD31
Gene ID (NCBI) 18613
RRIDAB_2919928
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ08481
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL594 CD31 antibody CL594-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols