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验证数据展示

  • FC experiment of mouse bone marrow cells using PE-65058

    FC experiment of mouse bone marrow cells using PE-65058

    1x10^6 mouse bone marrow cells were surface stained with 0.1 ug PE Anti-Mouse CD31 (PE-65058, Clone: 390) or 0.1 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were co-stained with APC Anti-Mouse CD45R (B220) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

PE Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for FC
Cat No. PE-65058

Print datasheet

CloneNo. 390

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

Pecam1, PECAM 1, Pecam, 390

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse bone marrow cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65058 targets CD31 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name platelet/endothelial cell adhesion molecule 1
Synonyms Pecam1, PECAM 1, Pecam, 390
GenBank Accession NumberBC008519
Gene Symbol CD31
Gene ID (NCBI) 18613
RRIDAB_2883855
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ08481
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
FC protocol for PE CD31 antibody PE-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols