验证数据展示
经过测试的应用
Positive IF/ICC detected in | bEnd.3 cells |
Positive FC detected in | mouse bone marrow cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
FC | See 3 publications below |
产品信息
FITC-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.
经测试应用 | IF/ICC, FC Application Description |
文献引用应用 | IF, FC |
经测试反应性 | mouse |
文献引用反应性 | mouse |
免疫原 | C3H/HeJ mouse hematopoietic progenitor cell line 32D 种属同源性预测 |
宿主/亚型 | Rat / IgG2a, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | platelet/endothelial cell adhesion molecule 1 |
别名 | PECAM-1, Pecam1, PECAM 1, Pecam, 390 |
GenBank蛋白编号 | BC008519 |
基因名称 | CD31 |
Gene ID (NCBI) | 18613 |
RRID | AB_2883748 |
偶联类型 | FITC Plus Fluorescent Dye |
最大激发/发射波长 | 495 nm / 524 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | Q08481 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
Product Specific Protocols | |
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IF protocol for FITC Plus CD31 antibody FITC-65058 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease. | ||
Bone mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification | ||
Cells Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage Polarization | ||
Biochim Biophys Acta Mol Cell Res CARM1 deficiency inhibits osteoblastic differentiation of bone marrow mesenchymal stem cells and delays osteogenesis in mice |