CD34 Monoclonal antibody
CD34 Monoclonal Antibody for WB, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3C8G12
验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue, human uterus tissue, human testis tissue |
Positive IHC detected in | human liver cancer tissue, human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human placenta tissue, human tonsillitis tissue, human liver cancer tissue, human liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 8 publications below |
FC | See 4 publications below |
产品信息
60180-1-Ig targets CD34 in WB, IF-P, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF-P, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD34 fusion protein Ag5996 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | CD34 molecule |
Synonyms | CD34 molecule, 3C8G12 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 105 kDa |
GenBank Accession Number | BC039146 |
Gene Symbol | CD34 |
Gene ID (NCBI) | 947 |
RRID | AB_10733337 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P28906 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD34 antibody 60180-1-Ig | Download protocol |
IHC protocol for CD34 antibody 60180-1-Ig | Download protocol |
IF protocol for CD34 antibody 60180-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Front Immunol Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation | ||
Front Bioeng Biotechnol Primary Repair for Treating Acute Proximal Anterior Cruciate Ligament Tears: A Histological Analysis and Prospective Clinical Trial. | ||
Exp Cell Res Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch. | ||
Biomed Mater The additive effects of photobiomodulation and bioactive glasses on enhancing early angiogenesis. | ||