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验证数据展示

  • IF Staining of human liver cancer using CL594-60180

    IF Staining of human liver cancer using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

CoraLite®594-conjugated CD34 Monoclonal antibody

CD34 Monoclonal Antibody for IF-P
Cat No. CL594-60180

Print datasheet

CloneNo. 3C8G12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Synonyms

CD34, CD34 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue, human tonsillitis tissue, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60180-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60180 targets CD34 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD34 fusion protein Ag5996 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD34 molecule
Synonyms CD34, CD34 molecule
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
RRIDAB_2883435
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28906
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
IF protocol for CL594 CD34 antibody CL594-60180Download protocol
Standard Protocols
Click here to view our Standard Protocols