CoraLite® Plus 488-conjugated CD34 Monoclonal antibody
CD34 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF-P and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C8G12
验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue, human placenta tissue |
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60180-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-60180 targets CD34 in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Immunogen | CD34 fusion protein Ag5996 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD34 molecule |
| Synonyms | CD34 molecule, 3C8G12 |
| Calculated Molecular Weight | 41 kDa |
| GenBank Accession Number | BC039146 |
| Gene Symbol | CD34 |
| Gene ID (NCBI) | 947 |
| RRID | AB_2883112 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28906 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD34 antibody CL488-60180 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |