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CoraLite® Plus 488-conjugated CD34 Monoclonal antibody

CD34 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C8G12

Cat no : CL488-60180

Print datasheet

Synonyms

CD34 molecule, 3C8G12

验证数据展示

  • IF Staining of human tonsillitis using CL488-60180

    IF Staining of human tonsillitis using CL488-60180

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-60180 (CD34 antibody), at dilution of 1:100.

  • IF Staining of human placenta using CL488-60180

    IF Staining of human placenta using CL488-60180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 CD34 antibody (CL488-60180, Clone: 3C8G12 ) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60180-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-60180 targets CD34 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Cited ApplicationsIF
Tested Reactivity human
Immunogen CD34 fusion protein Ag5996 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD34 molecule
Synonyms CD34 molecule, 3C8G12
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
RRIDAB_2883112
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28906
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD34 antibody CL488-60180Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Immunopharmacol Immunotoxicol

Fraxetin protects rat brains from the cerebral stroke via promoting angiogenesis and activating PI3K/Akt pathway.

Authors - Yuhuan Cui
View Article