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Species Reactivity
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验证数据展示

  • WB analysis of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    WB analysis of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

    IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

    IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD34 antibody included
  • IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 60180-1-Ig (same clone as 60180-1-PBS)

    IF Staining of human liver using 60180-1-Ig (same clone as 60180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta), ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

CD34 Monoclonal antibody, PBS Only

CD34 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60180-1-PBS

产品说明书

CloneNo. 3C8G12

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

CD34 molecule, 3C8G12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.

产品信息

60180-1-PBS targets CD34 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD34 fusion protein Ag5996 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD34 molecule
别名 CD34 molecule, 3C8G12
计算分子量 41 kDa
观测分子量 105 kDa
GenBank蛋白编号BC039146
基因名称 CD34
Gene ID (NCBI) 947
RRIDAB_10733337
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28906
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).