验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-65141 targets CD4 in FC applications and shows reactivity with mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | mouse |
| Immunogen | BALB/c mouse thymocytes 种属同源性预测 |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD4 antigen |
| Synonyms | T-cell surface antigen T4/Leu-3, T-cell differentiation antigen L3T4, RM4-5, Ly 4, L3T4 |
| GenBank Accession Number | BC039137 |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_2883022 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P06332 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD4 antibody APC-65141 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
