CoraLite® Plus 488-conjugated CD45 Recombinant antibody
CD45 Uni-rAbTM Recombinant Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6O19
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse spleen tissue |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80297-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80297 targets CD45 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | CD45 fusion protein Ag13712 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Synonyms | L CA, GP180, EC:3.1.3.48, CD45R, B220 |
| Calculated Molecular Weight | 1304 aa, 147 kDa |
| Observed Molecular Weight | 220-240 kDa |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45 antibody CL488-80297 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |