• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using CL555-65187

    FC experiment of human PBMCs using CL555-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 555 Anti-Human CD68 (CL555-65187, Clone: Y1/82A) (red) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL555-65187

    FC experiment of human PBMCs using CL555-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 555 Anti-Human CD68 (CL555-65187, Clone: Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 555 Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)
Cat No. CL555-65187

产品说明书

CloneNo. Y1/82A

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

Human

应用

FC (Intra)

别名

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65187-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 Lysosomal contents of lung macrophages 种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD68 molecule
别名 CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1
计算分子量 37 kDa
GenBank蛋白编号BC015557
基因名称 CD68
Gene ID (NCBI) 968
RRIDAB_3084583
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP34810
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD68 antibody CL555-65187Download protocol
Standard Protocols
Click here to view our Standard Protocols