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验证数据展示

  • FC experiment of human PBMCs using CL555-65187

    FC experiment of human PBMCs using CL555-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 555 Anti-Human CD68 (CL555-65187, Clone: Y1/82A) (red) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL555-65187

    FC experiment of human PBMCs using CL555-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 555 Anti-Human CD68 (CL555-65187, Clone: Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 555 Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)
Cat No. CL555-65187

Print datasheet

CloneNo. Y1/82A

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC (Intra)

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65187-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Lysosomal contents of lung macrophages 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD68 molecule
Synonyms CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
RRIDAB_3084583
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP34810
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD68 antibody CL555-65187Download protocol
Standard Protocols
Click here to view our Standard Protocols