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FITC Plus Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human and More (1)

Applications

FC (Intra) and More (1)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

Y1/82A

Cat no : FITC-65187

Print datasheet

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

验证数据展示

  • FC experiment of human PBMCs using FITC-65187

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

  • FC experiment of human PBMCs using FITC-65187

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

FITC-65187 targets CD68 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Lysosomal contents of lung macrophages 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD68 molecule
Synonyms CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP34810
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep Methods

Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids

Authors - Guoyun Bi
View Article