CoraLite® Plus 405 Anti-Mouse CD8a (53-6.7)
CD8a Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
53-6.7
验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65069-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65069 targets CD8a in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | Spleen cells or thymocyte membranes 种属同源性预测 |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD8 antigen, alpha chain |
| Synonyms | BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2 |
| GenBank Accession Number | BC030679 |
| Gene Symbol | Cd8a |
| Gene ID (NCBI) | 12525 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01731 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD8a antibody CL405-65069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
