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PerCP Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PerCP Fluorescent Dye

CloneNo.

53-6.7

Cat no : CP-65069

Print datasheet

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

验证数据展示

  • FC experiment of mouse splenocytes using CP-65069

    FC experiment of mouse splenocytes using CP-65069

    1x10^6 mouse splenocytes were surface stained with 0.25 ug PerCP Anti-Mouse CD8a (53-6.7) (CP-65069, Clone:53-6.7) or unstained. Cells were then stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CP-65069 targets CD8a in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Spleen cells or thymocyte membranes 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD8 antigen, alpha chain
Synonyms BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2
GenBank Accession NumberBC030679
Gene Symbol Cd8a
Gene ID (NCBI) 12525
Conjugate PerCP Fluorescent Dye
Excitation/Emission Maxima Wavelengths482 nm / 677 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01731
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
FC protocol for PerCP CD8a antibody CP-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols