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CoraLite® Plus 647 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

53-6.7

Cat no : CL647-65069

Print datasheet

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65069

    FC experiment of mouse splenocytes using CL647-65069

    1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65069, Clone:53-6.7) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65069

    IF Staining of mouse splenocytes using CL647-65069

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD8a (CL647-65069, Clone: 53-6.7) at dilution of 1:500.

经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65069-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65069 targets CD8a in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Mouse
Immunogen Spleen cells or thymocyte membranes 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD8 antigen, alpha chain
Synonyms BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2
GenBank Accession NumberBC030679
Gene Symbol Cd8a
Gene ID (NCBI) 12525
RRIDAB_2883664
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01731
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD8a antibody CL647-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols