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Anti-Human CD8b Rabbit Recombinant Antibody

CD8b Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241326A7

Cat no : 98103-1-RR

Print datasheet

Synonyms

CD8, 241326A7, Ly3, Leu2, CD8B1

验证数据展示

  • FC experiment of human PBMCs using 98103-1-RR

    FC experiment of human PBMCs using 98103-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were not fixed.

  • FC experiment of human PBMCs using 98103-1-RR

    FC experiment of human PBMCs using 98103-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7)(red), or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98103-1-RR

    Affinity and Kinetic Characterization of 98103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98103-1-RR against Human CD8b were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98103-1-RR targets CD8b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD8b molecule
Synonyms CD8, 241326A7, Ly3, Leu2, CD8B1
Calculated Molecular Weight 243 aa, 27 kDa
GenBank Accession NumberBC100913
Gene Symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10966
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

实验方案

Product Specific Protocols
FC protocol for CD8b antibody 98103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols