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PE Anti-Human CD8b Rabbit Recombinant Antibody

CD8b Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241326A7

Cat no : PE-98103

Print datasheet

Synonyms

CD8, 241326A7, Ly3, Leu2, CD8B1

验证数据展示

  • FC experiment of human PBMCs using PE-98103

    FC experiment of human PBMCs using PE-98103

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CD8b Rabbit Recombinant Antibody (PE-98103, Clone: 241326A7) or Isotype Control. Cells were not fixed.

  • FC experiment of human PBMCs using PE-98103

    FC experiment of human PBMCs using PE-98103

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CD8b Rabbit Recombinant Antibody (PE-98103, Clone: 241326A7) (red) or Isotype Control (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98103 targets CD8b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD8b molecule
Synonyms CD8, 241326A7, Ly3, Leu2, CD8B1
Calculated Molecular Weight 243 aa, 27 kDa
GenBank Accession NumberBC100913
Gene Symbol CD8B
Gene ID (NCBI) 926
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10966
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

实验方案

Product Specific Protocols
FC protocol for PE CD8b antibody PE-98103Download protocol
Standard Protocols
Click here to view our Standard Protocols