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验证数据展示

  • IF Staining of Caco-2 using CL488-82659

    IF Staining of Caco-2 using CL488-82659

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 CDX2 antibody (CL488-82659, Clone: 3J1 ) at dilution of 1:400.

  • IF Staining of Caco-2 using CL488-82659

    IF Staining of Caco-2 using CL488-82659

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 CDX2 antibody (CL488-82659, Clone: 3J1 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HepG2 using CL488-82659

    IF Staining of HepG2 using CL488-82659

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CDX2 antibody (CL488-82659, Clone: 3J1 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of Caco-2 using CL488-82659

    FC experiment of Caco-2 using CL488-82659

    1x10^6 Caco-2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CDX2 Recombinant antibody (CL488-82659, Clone:3J1)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CDX2 Recombinant antibody

CDX2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82659

产品说明书

CloneNo. 3J1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells, HepG2 cells
Positive FC (Intra) detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82659-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82659 targets CDX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 CDX2 fusion protein Ag17310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caudal type homeobox 2
别名 CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2
计算分子量 313 aa, 34 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC014461
基因名称 CDX2
Gene ID (NCBI) 1045
RRIDAB_3673070
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99626
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDX2 antibody CL488-82659Download protocol
FC protocol for CL Plus 488 CDX2 antibody CL488-82659Download protocol
Standard Protocols
Click here to view our Standard Protocols