• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

    IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

    IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using Collagen Type I antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • FC experiment of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    FC experiment of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    1x10^6 HSC-T6 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • FC experiment of SW480 using 83752-5-RR (same clone as 83752-5-PBS)

    FC experiment of SW480 using 83752-5-RR (same clone as 83752-5-PBS)

    1x10^6 SW480 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83752-5-RR

    Affinity and Kinetic Characterization of 83752-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-5-RR against Human COL1A2 were performed. The affinity constant is 6.77 nM.

Collagen Type I Recombinant antibody, PBS Only

Collagen Type I Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83752-5-PBS

产品说明书

CloneNo. 240665F8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83752-5-PBS targets Collagen Type I in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen
计算分子量 1366 aa, 130 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08123
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.