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验证数据展示

  • IHC staining of human skin using 83752-5-RR

    IHC staining of human skin using 83752-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin using 83752-5-RR

    IHC staining of human skin using 83752-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HSC-T6 using 83752-5-RR

    IF Staining of HSC-T6 using 83752-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HSC-T6 using 83752-5-RR

    IF Staining of HSC-T6 using 83752-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using Collagen Type I antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HSC-T6 using 83752-5-RR

    FC experiment of HSC-T6 using 83752-5-RR

    1x10^6 HSC-T6 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SW480 using 83752-5-RR

    FC experiment of SW480 using 83752-5-RR

    1x10^6 SW480 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83752-5-RR

    Affinity and Kinetic Characterization of 83752-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-5-RR against Human COL1A2 were performed. The affinity constant is 6.77 nM.

Collagen Type I Recombinant antibody

Collagen Type I Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83752-5-RR

产品说明书

CloneNo. 240665F8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHSC-T6 cells
Positive FC (Intra) detected inSW480 cells, HSC-T6 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83752-5-RR targets Collagen Type I in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen
计算分子量 1366 aa, 130 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
RRIDAB_3671349
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08123
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
IHC protocol for Collagen Type I antibody 83752-5-RRDownload protocol
IF protocol for Collagen Type I antibody 83752-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols