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验证数据展示

  • IF Staining of HSC-T6 using CL488-83752-5

    IF Staining of HSC-T6 using CL488-83752-5

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using CoraLite® Plus 488 Collagen Type I antibody (CL488-83752-5, Clone: 240665F8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Collagen Type I Recombinant antibody

Collagen Type I Recombinant Antibody for IF/ICC
Cat No. CL488-83752-5

产品说明书

CloneNo. 240665F8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC

别名

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHSC-T6 cells
For other applications, we recommend the unconjugated version of this antibody, 83752-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83752-5 targets Collagen Type I in IF/ICC applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen
计算分子量 1366 aa, 130 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08123
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Collagen Type I antibody CL488-83752-5Download protocol
Standard Protocols
Click here to view our Standard Protocols