CoraLite® Plus 488-conjugated Collagen Type I Recombinant antibody
Collagen Type I Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240665F8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HSC-T6 cells |
For other applications, we recommend the unconjugated version of this antibody, 83752-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83752-5 targets Collagen Type I in IF/ICC applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, rat |
| Immunogen | Collagen Type I fusion protein Ag6281 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | collagen, type I, alpha 2 |
| Synonyms | COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen |
| Calculated Molecular Weight | 1366 aa, 130 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC054498 |
| Gene Symbol | COL1A2 |
| Gene ID (NCBI) | 1278 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08123 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Collagen Type I antibody CL488-83752-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |