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验证数据展示

  • IF Staining of Hela using CL488-83089-2

    IF Staining of Hela using CL488-83089-2

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 COPA antibody (CL488-83089-2, Clone: 230361B1 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83089-2

    FC experiment of HeLa using CL488-83089-2

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated COPA Recombinant antibody (CL488-83089-2, Clone:230361B1)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated COPA Recombinant antibody

COPA Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83089-2

产品说明书

CloneNo. 230361B1

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IF/ICC

别名

Alpha coat protein, Alpha COP, Coatomer subunit alpha, COPA, FLJ26320, HEP COP, HEPCOP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy COPA Ready-To-Use IHC Kit. COPA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83089-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83089-2 targets COPA in IF/ICC applications and shows reactivity with Human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse
免疫原 COPA fusion protein Ag34207 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coatomer protein complex, subunit alpha
别名 Alpha coat protein, Alpha COP, Coatomer subunit alpha, COPA, FLJ26320, HEP COP, HEPCOP
计算分子量138 kDa
观测分子量150 kDa
GenBank蛋白编号NM_004371
基因名称 COPA
Gene ID (NCBI) 1314
RRIDAB_3673185
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53621
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 COPA antibody CL488-83089-2Download protocol
FC protocol for CL Plus 488 COPA antibody CL488-83089-2Download protocol
Standard Protocols
Click here to view our Standard Protocols