CUL1 Monoclonal antibody
CUL1 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B6D10
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NCI-H1299 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | human lung cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66978-1-Ig targets CUL1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | CUL1 fusion protein Ag28399 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | cullin 1 |
Synonyms | CUL 1, CUL1, cullin 1 |
Calculated Molecular Weight | 90 kDa |
Observed Molecular Weight | 85 kDa |
GenBank Accession Number | BC034318 |
Gene Symbol | CUL1 |
Gene ID (NCBI) | 8454 |
RRID | AB_2882298 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13616 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CUL1 antibody 66978-1-Ig | Download protocol |
IHC protocol for CUL1 antibody 66978-1-Ig | Download protocol |
IF protocol for CUL1 antibody 66978-1-Ig | Download protocol |
FC protocol for CUL1 antibody 66978-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |