• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CUL1 Monoclonal antibody, PBS Only

CUL1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1B6D10

Cat No : 66978-1-PBS

Print datasheet

Synonyms

CUL 1, CUL1, cullin 1

验证数据展示

  • WB analysis using 66978-1-Ig (same clone as 66978-1-PBS)

    WB analysis using 66978-1-Ig (same clone as 66978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66978-1-Ig (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66978-1-Ig (same clone as 66978-1-PBS)

    IHC staining of human lung cancer using 66978-1-Ig (same clone as 66978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66978-1-Ig (CUL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL1 antibody included
  • IF Staining of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

    IF Staining of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CUL1 antibody (66978-1-Ig, Clone: 1B6D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

    FC experiment of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (66978-1-Ig, Clone:1B6D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

产品信息

66978-1-PBS targets CUL1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CUL1 fusion protein Ag28399 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cullin 1
Synonyms CUL 1, CUL1, cullin 1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13616
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.