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验证数据展示

  • IF Staining of SH-SY5Y using CL555-66496

    IF Staining of SH-SY5Y using CL555-66496

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

  • IF Staining of human appendicitis using CL555-66496

    IF Staining of human appendicitis using CL555-66496

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

CoraLite®555-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL555-66496

Print datasheet

CloneNo. 2D7A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66496-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66496 targets Calretinin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms CALB2, calbindin 2, CAL2, CAB29, 2D7A9
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2919687
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

实验方案

Product Specific Protocols
IF protocol for CL555 Calretinin antibody CL555-66496Download protocol
Standard Protocols
Click here to view our Standard Protocols