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验证数据展示

  • WB analysis of mouse brain using HRP-66496

    WB analysis of mouse brain using HRP-66496

    mouse brain tissue were subjected to SDS PAGE followed by western blot with HRP-66496 (Calretinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HRP-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for WB
Cat No. HRP-66496

Print datasheet

CloneNo. 2D7A9

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-66496 targets Calretinin in WB applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, pig, rat
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms 29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2883843
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.This antibody is HRP conjugated.

实验方案

Product Specific Protocols
WB protocol for HRP Calretinin antibody HRP-66496Download protocol
Standard Protocols
Click here to view our Standard Protocols