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验证数据展示

  • WB analysis using 60555-1-Ig

    WB analysis using 60555-1-Ig

    Staurosporine treated and untreated A2780 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control.

  • IHC staining of Jurkat using 60555-1-Ig

    IHC staining of Jurkat using 60555-1-Ig

    Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Staurosporine treated Jurkat (right) cells slide using 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • IF Staining of HSC-T6 using 60555-1-Ig

    IF Staining of HSC-T6 using 60555-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HSC-T6 cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

  • FC experiment of HSC-T6 using 60555-1-Ig

    FC experiment of HSC-T6 using 60555-1-Ig

    1x10^6 HSC-T6 cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HSC-T6 cells (full lines) were intracellularly stained with 0.4 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

  • WB analysis using 60555-1-Ig

    WB analysis using 60555-1-Ig

    Staurosporine treated and untreated mouse splenocytes were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control.

  • IF Staining of HeLa using 60555-1-Ig

    IF Staining of HeLa using 60555-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HeLa cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:366 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • FC experiment of HeLa using 60555-1-Ig

    FC experiment of HeLa using 60555-1-Ig

    1x10^6 HeLa cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • WB analysis using 60555-1-Ig

    WB analysis using 60555-1-Ig

    Staurosporine treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control.

Cleaved PARP1 Monoclonal antibody

Cleaved PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 60555-1-Ig

产品说明书

CloneNo. 4G4C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA2780 cells, HSC-T6 cells, mouse splenocytes, Staurosporine treated A2780 cells, Staurosporine treated HSC-T6 cells, Staurosporine treated mouse splenocytes
Positive IHC detected inJurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in1 μM Staurosporine (3 hours) treated HSC-T6 cells, 1 μM Staurosporine (3 hours) treated HeLa cells
Positive FC (Intra) detected in1 μM  Staurosporine (3 hours) treated HSC-T6 cells, 1 μM  Staurosporine (3 hours) treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60555-1-Ig targets Cleaved PARP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 1
别名 PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT
计算分子量 1014 aa, 113 kDa
观测分子量89 kDa
GenBank蛋白编号BC037545
基因名称 PARP1
Gene ID (NCBI) 142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP09874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.

实验方案

Product Specific Protocols
WB protocol for Cleaved PARP1 antibody 60555-1-IgDownload protocol
IHC protocol for Cleaved PARP1 antibody 60555-1-IgDownload protocol
IF protocol for Cleaved PARP1 antibody 60555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols