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Cleaved PARP1 Monoclonal antibody, PBS Only (Capture)

Cleaved PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

4G4C8

Cat no : 60555-1-PBS

Print datasheet

Synonyms

PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

验证数据展示

  • WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    Staurosporine treated and untreated A2780 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 60555-1-Ig (same clone as 60555-1-PBS)

    IHC staining of Jurkat using 60555-1-Ig (same clone as 60555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Staurosporine treated Jurkat (right) cells slide using 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IF Staining of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

    IF Staining of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HSC-T6 cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • FC experiment of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

    FC experiment of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

    1x10^6 HSC-T6 cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HSC-T6 cells (full lines) were intracellularly stained with 0.4 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    Staurosporine treated and untreated mouse splenocytes were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

    IF Staining of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HeLa cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:366 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

    FC experiment of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

    1x10^6 HeLa cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

    Staurosporine treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

  • Sample test of MP50985-2

    Sample test of MP50985-2

    Cytometric bead array in cell lysate using MP50985-2, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-1-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.

产品信息

60555-1-PBS targets Cleaved PARP1 as part of a matched antibody pair:

MP50985-2: 60555-1-PBS capture and 66520-1-PBS detection (validated in N/A)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.