Dystrophin Recombinant antibody
Dystrophin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240567A3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83609-5-RR is Dystrophin Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Dystrophin fusion protein Ag3408 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | dystrophin |
Synonyms | DMD, BMD, 240567A3 |
Calculated Molecular Weight | 3685 aa, 427 kDa |
Observed Molecular Weight | 430 kDa, 70 kDa |
GenBank Accession Number | BC028720 |
Gene Symbol | Dystrophin |
Gene ID (NCBI) | 1756 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P11532 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Dystrophin antibody 83609-5-RR | Download protocol |
IHC protocol for Dystrophin antibody 83609-5-RR | Download protocol |
IF protocol for Dystrophin antibody 83609-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |