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验证数据展示

  • IF Staining of HepG2 using CL488-66655

    IF Staining of HepG2 using CL488-66655

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 EIF4E antibody (CL488-66655, Clone: 3C6B9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66655

    FC experiment of HepG2 using CL488-66655

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF4E (CL488-66655, Clone:3C6B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EIF4E Monoclonal antibody

EIF4E Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66655

产品说明书

CloneNo. 3C6B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66655-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66655 targets EIF4E in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 EIF4E fusion protein Ag27191 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E
别名 eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E
计算分子量 29 kDa
观测分子量 25 kDa
GenBank蛋白编号BC012611
基因名称 EIF4E
Gene ID (NCBI) 1977
RRIDAB_2883364
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06730
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF4E antibody CL488-66655Download protocol
FC protocol for CL Plus 488 EIF4E antibody CL488-66655Download protocol
Standard Protocols
Click here to view our Standard Protocols