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CoraLite®594-conjugated EIF4E Monoclonal antibody
EIF4E Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3C6B9
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66655-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66655 targets EIF4E in IF applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | EIF4E fusion protein Ag27191 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 4E |
Synonyms | CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC012611 |
Gene Symbol | EIF4E |
Gene ID (NCBI) | 1977 |
RRID | AB_2883611 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P06730 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 EIF4E antibody CL594-66655 | Download protocol |
FC protocol for CL594 EIF4E antibody CL594-66655 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |