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- KD/KO Validated
CoraLite® Plus 647-conjugated EIF4E Monoclonal antibody
EIF4E Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3C6B9
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66655-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66655 targets EIF4E in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | EIF4E fusion protein Ag27191 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 4E |
| Synonyms | eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC012611 |
| Gene Symbol | EIF4E |
| Gene ID (NCBI) | 1977 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P06730 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 EIF4E antibody CL647-66655 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
