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验证数据展示

  • IF Staining of HepG2 using CL488-81504

    IF Staining of HepG2 using CL488-81504

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ENO1/2/3 antibody (CL488-81504, Clone: 6E24 ) at dilution of 1:400.

  • FC experiment of HepG2 using CL488-81504

    FC experiment of HepG2 using CL488-81504

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ENO1/2/3 Recombinant antibody (CL488-81504, Clone:6E24)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ENO1/2/3 Recombinant antibody

ENO1/2/3 Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-81504

产品说明书

CloneNo. 6E24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81504-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81504 targets ENO1/2/3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ENO1/2/3 fusion protein Ag1692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enolase 1, (alpha)
别名 ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC015641
基因名称 ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
RRIDAB_3673046
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06733
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ENO1/2/3 antibody CL488-81504Download protocol
FC protocol for CL Plus 488 ENO1/2/3 antibody CL488-81504Download protocol
Standard Protocols
Click here to view our Standard Protocols