验证数据展示

WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11257-1-AP
WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 11257-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11257-1-AP
IP result of anti-ERK1/2 (IP:11257-1-AP, 4ug; Detection:11257-1-AP 1:3000) with HEK-293 cells lysate 1440 ug.
IF Staining of HEK-293 using 11257-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ERK1/2 antibody (11257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11257-1-AP

产品说明书

引用文献(499)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (499)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	PC-12 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 485 publications below
IHC	See 11 publications below
IF	See 15 publications below
CoIP	See 2 publications below

产品信息

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, canine, chicken, zebrafish, bovine, sheep, goat
免疫原	CatNo: Ag1759 Product name: Recombinant human ERK1/2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-379 aa of BC013992 Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	mitogen-activated protein kinase 3
别名	ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1
计算分子量	43 kDa
观测分子量	38-44 kDa
GenBank蛋白编号	BC013992
基因名称	ERK1
Gene ID (NCBI)	5595
RRID	AB_2139822
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P27361
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
IF protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IHC protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IP protocol for ERK1/2 antibody 11257-1-AP	Download protocol
WB protocol for ERK1/2 antibody 11257-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer Authors - Yurou Chen View Article
mouse	WB	Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure Authors - Lingjun Tong View Article
mouse	WB	Small Biomimetic Nanoplatform Integrating CeO₂ Nanozymes and Anti-Inflammatory Peptides for Osteoarthritis Therapy Authors - Chengli Yang View Article
human	WB	Redox Biol Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species Authors - He Zhao View Article
	WB	Cancer Res Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells Authors - Li Xia View Article
mouse	WB	EMBO Mol Med Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice Authors - Huiting Wang View Article

验证数据展示

WB analysis using 11257-1-AP

WB analysis of HEK-293 using 11257-1-AP

IHC staining of human colon cancer using 11257-1-AP

IHC staining of human colon cancer using 11257-1-AP

FC experiment of HeLa using 11257-1-AP

WB analysis using 11257-1-AP

IP experiment of HEK-293 using 11257-1-AP

IF Staining of HEK-293 using 11257-1-AP

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure

Biomimetic Nanoplatform Integrating CeO₂ Nanozymes and Anti-Inflammatory Peptides for Osteoarthritis Therapy

Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species

Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells

Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice