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ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11257-1-AP

Print datasheet

Synonyms

ERK1, ERK, ERK2, ERT2, ERK-1



经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, sheep
Immunogen ERK1/2 fusion protein Ag1759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 3
Synonyms ERK1, ERK, ERK2, ERT2, ERK-1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
RRIDAB_2139822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27361
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
WB protocol for ERK1/2 antibody 11257-1-APDownload protocol
IHC protocol for ERK1/2 antibody 11257-1-APDownload protocol
IF protocol for ERK1/2 antibody 11257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Authors - Yurou Chen
humanWB

Redox Biol

Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species

Authors - He Zhao
WB

Cancer Res

Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells

Authors - Li Xia
mouseWB

ACS Appl Mater Interfaces

Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.

Authors - Chaoqun Li
mouseWB

J Nanobiotechnology

Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis

Authors - Ni Yang
humanWB

J Nanobiotechnology

ACYP2 functions as an innovative nano-therapeutic target to impede the progression of hepatocellular carcinoma by inhibiting the activity of TERT and the KCNN4/ERK pathway

Authors - Yixuan Wu