- Featured Product
- KD/KO Validated
CoraLite® Plus 555-conjugated ERK1/2 Polyclonal antibody
ERK1/2 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 11257-1-AP
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-11257 targets ERK1/2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ERK1/2 fusion protein Ag1759 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase 3 |
Synonyms | ERK1, ERK, ERK2, ERT2, ERK-1 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 38-44 kDa |
GenBank Accession Number | BC013992 |
Gene Symbol | ERK1 |
Gene ID (NCBI) | 5595 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P27361 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERK1 and ERK2 belong to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 555 ERK1/2 antibody CL555-11257 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |