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验证数据展示

  • FC experiment of HepG2 using CL647-11257

    FC experiment of HepG2 using CL647-11257

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ERK1/2 (CL647-11257) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for FC (Intra)
Cat No. CL647-11257

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

ERK1, ERK, ERK2, ERT2, ERK-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11257-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11257 targets ERK1/2 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1759

Product name: Recombinant human ERK1/2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-379 aa of BC013992

Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 3
别名 ERK1, ERK, ERK2, ERT2, ERK-1
计算分子量 43 kDa
观测分子量 38-44 kDa
GenBank蛋白编号BC013992
基因名称 ERK1
Gene ID (NCBI) 5595
RRIDAB_2934853
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27361
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ERK1/2 antibody CL647-11257Download protocol
Standard Protocols
Click here to view our Standard Protocols