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验证数据展示

  • FC experiment of HeLa using CL488-11257

    FC experiment of HeLa using CL488-11257

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ERK1/2 (CL488-11257) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-11257

    IF Staining of MCF-7 using CL488-11257

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ERK1/2 antibody (CL488-11257) at dilution of 1:500.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11257

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

ERK1, ERK, ERK2, ERT2, ERK-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11257-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11257 targets ERK1/2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen ERK1/2 fusion protein Ag1759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 3
Synonyms ERK1, ERK, ERK2, ERT2, ERK-1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
RRIDAB_2919021
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27361
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ERK1/2 antibody CL488-11257Download protocol
FC protocol for CL Plus 488 ERK1/2 antibody CL488-11257Download protocol
Standard Protocols
Click here to view our Standard Protocols