验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 83447-3-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83447-3 targets ESR2 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | ESR2 fusion protein Ag5103 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | estrogen receptor 2 (ER beta) |
| Synonyms | ESTRB, ERβ, ER-beta, Erb, ER BETA |
| Calculated Molecular Weight | 59 kDa |
| GenBank Accession Number | BC024181 |
| Gene Symbol | ESR2 |
| Gene ID (NCBI) | 2100 |
| RRID | AB_3673254 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92731 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ESR2 antibody CL488-83447-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |