CoraLite® Plus 488-conjugated ESR2 Recombinant antibody

ESR2 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83447-3

产品说明书

CloneNo. 240412F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

ESTRB, ERβ, ER-beta, Erb, ER BETA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83447-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83447-3 targets ESR2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 ESR2 fusion protein Ag5103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 ESTRB, ERβ, ER-beta, Erb, ER BETA
计算分子量 59 kDa
GenBank蛋白编号BC024181
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_3673254
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92731
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ESR2 antibody CL488-83447-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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