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验证数据展示

  • WB analysis of HeLa using 66629-1-Ig

    WB analysis of HeLa using 66629-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66629-1-Ig (FAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66629-1-Ig

    IHC staining of human breast cancer using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 66629-1-Ig

    WB analysis of HeLa using 66629-1-Ig

    WB result of FAF2 antibody (66629-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66629-1-Ig
CloneNo. 3H6D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66629-1-Ig targets FAF2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen FAF2 fusion protein Ag21536 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fas associated factor family member 2
Synonyms ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B
Calculated Molecular Weight 445 aa, 53 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
RRIDAB_2881989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

实验方案

Product Specific Protocols
WB protocol for FAF2 antibody 66629-1-IgDownload protocol
IHC protocol for FAF2 antibody 66629-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols