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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H6D2

Cat no : CL594-66629

Print datasheet

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

验证数据展示

  • IF Staining of HEK-293 using CL594-66629

    IF Staining of HEK-293 using CL594-66629

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 FAF2 antibody (CL594-66629, Clone: 3H6D2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66629-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66629 targets FAF2 in IF/ICC applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse
Immunogen FAF2 fusion protein Ag21536 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fas associated factor family member 2
Synonyms ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B
Calculated Molecular Weight 445 aa, 53 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CS3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

实验方案

Product Specific Protocols
IF protocol for CL594 FAF2 antibody CL594-66629Download protocol
Standard Protocols
Click here to view our Standard Protocols