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CoraLite® Plus 488-conjugated FAF2 Monoclonal antibody
FAF2 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3H6D2
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66629-1-Ig
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66629 targets FAF2 in WB applications and shows reactivity with human, mouse samples.
Tested Applications | WB Application Description |
Tested Reactivity | human, mouse |
Immunogen | FAF2 fusion protein Ag21536 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | Fas associated factor family member 2 |
Synonyms | ETEA, 3H6D2, Protein ETEA, KIAA0887, FAS-associated factor 2 |
Calculated Molecular Weight | 445 aa, 53 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC014001 |
Gene Symbol | FAF2 |
Gene ID (NCBI) | 23197 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q96CS3 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 488 FAF2 antibody CL488-66629 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |