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验证数据展示

  • WB analysis using CL488-66629

    WB analysis using CL488-66629

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66629 (FAF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for WB
Cat No. CL488-66629

产品说明书

CloneNo. 3H6D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB

别名

ETEA, 3H6D2, Protein ETEA, KIAA0887, FAS-associated factor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66629-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66629 targets FAF2 in WB applications and shows reactivity with human, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, mouse
免疫原 FAF2 fusion protein Ag21536 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Fas associated factor family member 2
别名 ETEA, 3H6D2, Protein ETEA, KIAA0887, FAS-associated factor 2
计算分子量 445 aa, 53 kDa
观测分子量 51 kDa
GenBank蛋白编号BC014001
基因名称 FAF2
Gene ID (NCBI) 23197
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96CS3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 FAF2 antibody CL488-66629Download protocol
Standard Protocols
Click here to view our Standard Protocols