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CoraLite® Plus 488-conjugated FDX1 Recombinant antibody

FDX1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230196B12

Cat No : CL488-82957

Print datasheet

Synonyms

Ferredoxin-1, FDX, ADX, 230196B12

验证数据展示

  • IF Staining of HepG2 using CL488-82957

    IF Staining of HepG2 using CL488-82957

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FDX1 antibody (CL488-82957, Clone: 230196B12 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-82957

    FC experiment of A431 using CL488-82957

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fdx1 Recombinant Antibody (CL488-82957, Clone:230196B12)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inA431 cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82957-1-RR

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82957 targets FDX1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen FDX1 fusion protein Ag3301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ferredoxin 1
Synonyms Ferredoxin-1, FDX, ADX, 230196B12
Calculated Molecular Weight 184 aa, 19 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC017063
Gene Symbol FDX1
Gene ID (NCBI) 2230
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10109
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FDX1 antibody CL488-82957Download protocol
FC protocol for CL Plus 488 FDX1 antibody CL488-82957Download protocol
Standard Protocols
Click here to view our Standard Protocols