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Validation Data Gallery

  • Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

    Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

ChromoTek GFP-Booster ATTO647N

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Cat No. gba647n
引用文献(69)

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

ATTO 647N

Host

Alpaca

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Formats:  ATTO 647N
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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

DescriptionThe GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetCFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateATTO 647N
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:200
FormatAlpaca single domain antibody, Nanobody or VHH; monovalent
Type Nanobody
ClassRecombinant
RRIDAB_2629215
Affinity (KD) Dissociation constant KD of 1 pM
Storage Buffer1x PBS, 0.09% sodium azide
Excitation/ EmissionExcitation max: 647 nm, Emission max: 669 nm
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle

Nat Methods

Virtual reality-empowered deep-learning analysis of brain cells

Authors - Doris Kaltenecker
View Article
IF

Nature

A trans-synaptic nanocolumn aligns neurotransmitter release to receptors.

Authors - Ai-Hui Tang
View Article
IF

Cell

Cellular and Molecular Probing of Intact Human Organs.

Authors - Shan Zhao
View Article
IF

Cell

Physical Principles of Membrane Shape Regulation by the Glycocalyx.

Authors - Carolyn R Shurer
View Article
IF

Cell

Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body.

Authors - Chenchen Pan
View Article
IF

Nat Neurosci

Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections.

Authors - Ruiyao Cai
View Article