Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
| Description | The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:
• Considerably higher tissue penetration rates • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Less than 2 nm epitope-label displacement minimizes linkage error • Monovalent VHHs do not cluster their epitopes • Validation: structure and function characterized • Consistent and reliable performance due to recombinant production |
| Applications | IF, IHC, ICC |
| Host | Alpaca |
| Specificity/Target | CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet For the complete list, please click here: Fluorescent protein specificity table Nano-Booster |
| Conjugate | Alexa Fluor® 647 |
| Physical State | Liquid |
| Suggested Dilution | IF/ICC/IHC: 1:500- 1:1,000 |
| Type | Nanobody |
| Class | Recombinant |
| DOL | 2 fluorophores per Nanobody |
| Format | Alpaca single domain antibody, Nanobody or VHH; monovalent |
| RRID | AB_2827575 |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Excitation/ Emission | Excitation max: 650 nm, Emission max: 665 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light. |
| Size | 10 μL; 50 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| gb2AF647_SDS_GFP-Booster Alexa Fluor® 647 (EN) |
| Datasheet |
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| GFP-Booster Alexa Fluor® 647 Datasheet |
| GFP-TRAP Specificity |
|---|
| Fluorescent protein specificity table Nano-Booster |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nat Methods Varying label density allows artifact-free analysis of membrane-protein nanoclusters. | |
Sci Adv Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging. | |
Nat Commun Extent of myosin penetration within the actin cortex regulates cell surface mechanics. | |
EMBO J Chromatin stiffening underlies enhanced locus mobility after DNA damage in budding yeast. | |