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ChromoTek GFP-Booster Alexa Fluor® 488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Host

Alpaca

Cat No : gb2AF488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Validation Data Gallery

  • Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

DescriptionThe GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetCFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateAlexa Fluor® 488
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:500- 1:1,000
Type Nanobody
ClassRecombinant
DOL2 fluorophores per Nanobody
RRIDAB_2827573
Affinity (KD) Dissociation constant KD of 1 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Nat Biotechnol

Three-dimensional structured illumination microscopy with enhanced axial resolution

Authors - Xuesong Li
View Article
IF

Nat Cell Biol

CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry.

Authors - Nan-Peng Chen
View Article
IF

Mol Cell

Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.

Authors - Marion E Pesenti
View Article

Nucleic Acids Res

COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans

Authors - Yuejun Yang
View Article
IF

Nat Commun

Actin nano-architecture of phagocytic podosomes

Authors - J. Cody Herron
View Article
IF

J Exp Med

Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesis.

Authors - René Rauschmeier
View Article