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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL488-66323

Print datasheet

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

验证数据展示

  • IF Staining of mouse brain using CL488-66323

    IF Staining of mouse brain using CL488-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66323 (Glutamine synthetase antibody) at dilution of 1:50.

  • FC experiment of HepG2 using CL488-66323

    FC experiment of HepG2 using CL488-66323

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Glutamine Synthetase (CL488-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66323-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66323 targets Glutamine Synthetase in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
GenBank Accession NumberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2883309
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Glutamine Synthetase antibody CL488-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols