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CoraLite® Plus 488-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

IF-P, FC (Intra) and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL488-66323

Print datasheet

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59



经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66323-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66323 targets Glutamine Synthetase in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity Human, Mouse, Rat
Cited Reactivityzebrafish
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
GenBank Accession NumberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2883309
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Glutamine Synthetase antibody CL488-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
zebrafishIF

J Cell Sci

Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types

Authors - Aanandita A Kothurkar