CoraLite® Plus 750-conjugated HLA-DMA Recombinant antibody

HLA-DMA Uni-rAbTM Recombinant Antibody for WB
Cat No. CL750-82899-2

产品说明书

CloneNo. 230155E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB

hCG_2001572, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, DMA protein, DMA, D6S222E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inRaji cells, Ramos cells
For other applications, we recommend the unconjugated version of this antibody, 82899-2-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82899-2 targets HLA-DMA in WB applications and shows reactivity with human samples.

经测试应用 WB Application Description
经测试反应性 human
免疫原 HLA-DMA fusion protein Ag2452 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DM alpha
别名 hCG_2001572, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, DMA protein, DMA, D6S222E
计算分子量 261 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC026279
基因名称 HLA-DMA
Gene ID (NCBI) 3108
RRIDAB_3673744
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ31604
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 HLA-DMA antibody CL750-82899-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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