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Human IgG4 Monoclonal antibody, PBS Only

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat No : 66408-1-PBS

Print datasheet

Synonyms

Human IgG4, IgG4, IGHG4

验证数据展示

  • IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

    WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

产品信息

66408-1-PBS targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Synonyms Human IgG4, IgG4, IGHG4
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.