• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Human IgG4 Monoclonal antibody, PBS Only

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat no : 66408-1-PBS

Print datasheet

Synonyms

Human IgG4, IgG4, IGHG4

验证数据展示

  • IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

    WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

    IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

产品信息

66408-1-PBS targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Synonyms Human IgG4, IgG4, IGHG4
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2881780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.