• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human tonsillitis using 66408-1-Ig

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using 66408-1-Ig

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • WB analysis of human plasma using 66408-1-Ig

    WB analysis of human plasma using 66408-1-Ig

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IF Staining of human appendicitis using 66408-1-Ig

    IF Staining of human appendicitis using 66408-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66408-1-Ig

产品说明书

CloneNo. 1A1A2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Human IgG4, IgG4, IGHG4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66408-1-Ig targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin heavy constant gamma 4 (G4m marker)
别名 Human IgG4, IgG4, IGHG4
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号AL928742
基因名称 IGHG4
Gene ID (NCBI) 3503
RRIDAB_2881780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

实验方案

Product Specific Protocols
WB protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IHC protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IF protocol for Human IgG4 antibody 66408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Breast Cancer Res

Clinical and biological heterogeneities in triple-negative breast cancer reveals a non-negligible role of HER2-low

Authors - Xi E Hu
View Article