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Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for WB, IF-P, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF-P, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat No : 66408-1-Ig

Print datasheet

Synonyms

Human IgG4, IgG4, IGHG4

验证数据展示

  • IHC staining of human tonsillitis using 66408-1-Ig

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • IHC staining of human tonsillitis using 66408-1-Ig

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG4 antibody included
  • WB analysis of human plasma using 66408-1-Ig

    WB analysis of human plasma using 66408-1-Ig

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IF Staining of human appendicitis using 66408-1-Ig

    IF Staining of human appendicitis using 66408-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66408-1-Ig targets Human IgG4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF-P, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Synonyms Human IgG4, IgG4, IGHG4
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2881780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

实验方案

Product Specific Protocols
WB protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IHC protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IF protocol for Human IgG4 antibody 66408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Breast Cancer Res

Clinical and biological heterogeneities in triple-negative breast cancer reveals a non-negligible role of HER2-low

Authors - Xi E Hu
View Article